![]() However, miR-143 inhibition repressed phosphorylation of Erk5 and abrogated cyclin D1 with concomitant reduction in cells entering cell cycle. FoxO1-specific siRNA upregulated miR-143, whereas inhibition of miR-143 did not change FoxO1 activation. Luciferase assay indicated a dependent relationship between miR-143 and Erk5 in AAMSC. miR array supported by real-time PCR showed induction of miR-143 in AAMSC (4.73-fold vs. Flow cytometry showed > 10% higher S-phase cell population that was confirmed by BrdU assay (15%) and immunohistology for Ki67 (11%) in AAMSC using EmpMSC as controls. AAMSC had higher phosphorylation of FoxO1, which activated Erk5, a distinct mitogen-induced MAPK that drove transcriptional activation of cyclin D1 and Cdk4. Mesenchymal stem cells (MSC) from young male rats were transduced with Ad-vectors encoding for Akt ( AktMSC) and Ang-1 ( Ang-1MSC) transgenes for their individual or simultaneous overexpression ( AAMSC > 5-fold gene level and > 4-fold Akt and Ang-1 protein expression in AAMSC vs. We report that simultaneous expression of Akt and angiopoietin-1 (Ang-1) transgenes supported mitogenesis in stem cells with a critical role for microRNA-143 (miR-143) downstream of FoxO1 transcription factor. ![]()
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